HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Improved endothelialization and reduced thrombosis by coating a synthetic vascular graft with fibronectin and stem cell homing factor SDF-1α.

Abstract
Failure of synthetic small-diameter vascular grafts is determined mainly by the lack of endothelial cells, as these cells inhibit thrombosis and intimal hyperplasia. Coating of graft material with homing factors for circulating stem cells has the potential to improve endogenous endothelialization of these grafts and to reduce graft failure. Synthetic knitted polyester grafts (6mm diameter) were coated with FN and SDF-1α before surgical interposition in the carotid artery of sheep. Similar uncoated vascular grafts were implanted in the contralateral side as internal controls. To study the early attraction of stem cells, grafts were implanted in a first series of nine sheep and explanted after 1 or 3 days. In coated grafts, four times higher fractions of CD34(+) and three to four times higher fractions of CD117(+) cells adhering to the vessel walls were found than in control grafts (P<0.05). When such coated and non-coated grafts were implanted in 12 other sheep and explanted after 3 months, all coated grafts were patent, while one control graft was occluded. EcNOS staining revealed that FN-SDF-1α coating significantly increased coverage with endothelial cells from 27 ± 4% of the graft to 48 ± 4% compared with the controls (P=0.001). This was associated with a significant reduction of intimal hyperplasia (average thickness 1.03 ± 0.09 mm in controls vs. 0.69 ± 0.04 mm in coated grafts; P=0.009) and significantly less adhesion of thrombotic material in the middle part of the graft (P=0.029). FN-SDF-1α coating of synthetic small-caliber vascular grafts stimulated the attraction of stem cells and was associated with improved endothelialization and reduced intimal hyperplasia and thrombosis.
AuthorsG De Visscher, L Mesure, B Meuris, A Ivanova, W Flameng
JournalActa biomaterialia (Acta Biomater) Vol. 8 Issue 3 Pg. 1330-8 (Mar 2012) ISSN: 1878-7568 [Electronic] England
PMID21964214 (Publication Type: Journal Article)
CopyrightCopyright © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Chemical References
  • Chemokine CXCL12
  • Coated Materials, Biocompatible
  • Fibronectins
Topics
  • Animals
  • Blood Vessel Prosthesis
  • Carotid Arteries
  • Cell Adhesion
  • Chemokine CXCL12 (chemistry)
  • Coated Materials, Biocompatible (adverse effects, chemistry)
  • Endothelial Cells (metabolism, pathology)
  • Female
  • Fibronectins (chemistry)
  • Materials Testing
  • Sheep
  • Stem Cells (metabolism, pathology)
  • Thrombosis (etiology, prevention & control)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: