In vivo gene transfer into the ocular ciliary muscle mediated by ultrasound and microbubbles.
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| Abstract |
This study aimed to assess application of ultrasound (US) combined with microbubbles (MB) to transfect the ciliary muscle of rat eyes. Reporter DNA plasmids encoding for Gaussia luciferase, β-galactosidase or the green fluorescent protein (GFP), alone or mixed with 50% Artison MB, were injected into the ciliary muscle, with or without US exposure (US set at 1 MHz, 2 W/cm(2), 50% duty cycle for 2 min). Luciferase activity was measured in ocular fluids at 7 and 30 days after sonoporation. At 1 week, the US+MB treatment showed a significant increase in luminescence compared with control eyes, injected with plasmid only, with or without MB (×2.6), and, reporter proteins were localized in the ciliary muscle by histochemical analysis. At 1 month, a significant decrease in luciferase activity was observed in all groups. A rise in lens and ciliary muscle temperature was measured during the procedure but did not result in any observable or microscopic damages at 1 and 8 days. The feasibility to transfer gene into the ciliary muscle by US and MB suggests that sonoporation may allow intraocular production of proteins for the treatment of inflammatory, angiogenic and/or degenerative retinal diseases.
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| Authors | Laura Kowalczuk, Michèle Boudinet, Mohamed El Sanharawi, Elodie Touchard, Marie-Christine Naud, Amena Saïed, Jean-Claude Jeanny, Francine Behar-Cohen, Pascal Laugier |
| Journal | Ultrasound in medicine & biology (Ultrasound Med Biol) Vol. 37 Issue 11 Pg. 1814-27 (Nov 2011) ISSN: 1879-291X [Electronic] England |
| PMID | 21963032 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2011 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved. |
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