HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

[Identification of Mycobacterium avium ssp. paratuberculosis infected dairy herds by environmental sampling].

Abstract
In herds with known prevalence (P) use of environmental sampling (ES) to detect Mycobacterium avium ssp. paratuberculosis (MAP) infected cattle herds was proofed in relation to P. In 31 MAP-infected free stall dairy herds and 15 non-infected herds P was defined by annually repeated whole herd testing by fecal culture (34 877 individual samples). Eight infected herds had a very low (> 0-2%), 14 a low (> 2-5%), four a medium (> 5-10%), and five a high P (> 10%). A mean number of nine environmental samples per herd were collected from the floor of lactating cows, milking, calving and sick cow areas and the crossover to the calf area. After twelve weeks cultivation on HEYM-medium with and without mycobactin positive samples were further characterized by PCR. All non-infected herds (100%) showed negative and 22 (71%) of the infected herds positive results in ES. Nine infected herds with negative ES results had a low P (0.04-4,04%). Proportion of positive ES depended on P and on sampling areas with 53.3% positive results in lactating cow areas and 45.2% in milking areas. For P > 5%, ES in these two areas caused a positive herd status; herds with P < 5% required sampling in the other areas too. The ES method has a herd sensitivity of 87% for dairy herds with P > 2% and provides an efficient tool to determine MAP infection status or herd prevalence.
AuthorsKarsten Donat, Ute Schau, Anneka Soschinka
JournalBerliner und Munchener tierarztliche Wochenschrift (Berl Munch Tierarztl Wochenschr) 2011 Sep-Oct Vol. 124 Issue 9-10 Pg. 360-7 ISSN: 0005-9366 [Print] Germany
Vernacular TitleIdentifizierung von mit Mycobacterium avium ssp. paratuberculosis (MAP) infi- zierten Milchviehbeständen mithilfe von Umgebungskotproben.
PMID21950212 (Publication Type: English Abstract, Journal Article)
Topics
  • Animals
  • Cattle
  • Cattle Diseases (epidemiology, microbiology)
  • Dairying
  • Environmental Microbiology
  • Feces (microbiology)
  • Female
  • Germany (epidemiology)
  • Housing, Animal
  • Male
  • Mycobacterium avium subsp. paratuberculosis (isolation & purification)
  • Paratuberculosis (epidemiology, microbiology)
  • Prevalence

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: