Abstract |
Truncated recombinant nucleocapsid proteins (trNs) that lack N-terminally located cross-reactive epitopes of four Murinae rodent-associated hantaviruses, Seoul virus (SEOV), Thailand virus, Hantaan virus (HTNV) and Dobrava-Belgrade virus, were produced by using a baculovirus expression system. ELISA with the trNs as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses with cut-off OD values of 60% of those of whole N of HTNV. The trN-based ELISA could serotype 12 out of 13 sera obtained from wild rodents (Rattus norvegicus) naturally infected with SEOV using the 60% cut-off value. These results indicate that screening with whole N followed by serotyping with trNs using a cut-off OD value of 60% of that of whole N is a useful method for serological surveillance of Murinae-associated hantavirus infection among rodents.
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Authors | Shumpei P Yasuda, Kumiko Yoshimatsu, Takaaki Koma, Kenta Shimizu, Rika Endo, Rie Isozumi, Jiro Arikawa |
Journal | The Journal of veterinary medical science
(J Vet Med Sci)
Vol. 74
Issue 2
Pg. 215-9
(Feb 2012)
ISSN: 1347-7439 [Electronic] Japan |
PMID | 21937858
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunoglobulin G
- Nucleocapsid Proteins
- Recombinant Proteins
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Topics |
- Animals
- Enzyme-Linked Immunosorbent Assay
(methods)
- Orthohantavirus
(classification, genetics)
- Hantavirus Infections
(diagnosis, virology)
- Immunoglobulin G
(analysis)
- Nucleocapsid Proteins
(chemistry, genetics)
- Rats
- Recombinant Proteins
(chemistry)
- Serotyping
(methods)
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