HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Application of truncated nucleocapsid protein (N) for serotyping ELISA of murinae-associated hantavirus infection in rats.

Abstract
Truncated recombinant nucleocapsid proteins (trNs) that lack N-terminally located cross-reactive epitopes of four Murinae rodent-associated hantaviruses, Seoul virus (SEOV), Thailand virus, Hantaan virus (HTNV) and Dobrava-Belgrade virus, were produced by using a baculovirus expression system. ELISA with the trNs as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses with cut-off OD values of 60% of those of whole N of HTNV. The trN-based ELISA could serotype 12 out of 13 sera obtained from wild rodents (Rattus norvegicus) naturally infected with SEOV using the 60% cut-off value. These results indicate that screening with whole N followed by serotyping with trNs using a cut-off OD value of 60% of that of whole N is a useful method for serological surveillance of Murinae-associated hantavirus infection among rodents.
AuthorsShumpei P Yasuda, Kumiko Yoshimatsu, Takaaki Koma, Kenta Shimizu, Rika Endo, Rie Isozumi, Jiro Arikawa
JournalThe Journal of veterinary medical science (J Vet Med Sci) Vol. 74 Issue 2 Pg. 215-9 (Feb 2012) ISSN: 1347-7439 [Electronic] Japan
PMID21937858 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Immunoglobulin G
  • Nucleocapsid Proteins
  • Recombinant Proteins
Topics
  • Animals
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Orthohantavirus (classification, genetics)
  • Hantavirus Infections (diagnosis, virology)
  • Immunoglobulin G (analysis)
  • Nucleocapsid Proteins (chemistry, genetics)
  • Rats
  • Recombinant Proteins (chemistry)
  • Serotyping (methods)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: