Study of the enhanced anticancer efficacy of gambogic acid on Capan-1 pancreatic cancer cells when mediated via magnetic Fe3O4 nanoparticles.

Abstract

BACKGROUND:

Gambogic acid (GA), a potent anticancer agent, is limited in clinical administration due to its poor water solubility. The aim of this study was to explore a drug delivery system based on magnetic Fe(3)O(4) nanoparticles (MNP-Fe(3)O(4)) conjugated with GA to increase water solubility of the drug and enhance its chemotherapeutic efficiency for pancreatic cancer.

METHODS:

GA was conjugated with the MNP-Fe(3)O(4) colloidal suspension by mechanical absorption polymerization to construct GA-loaded MNP-Fe(3)O(4), which acted as a drug delivery system.

RESULTS:

Combination therapy with GA and MNP-Fe(3)O(4) induced remarkable improvement in anticancer activity, which was demonstrated by optical microscopic observations, MTT assay, and nuclear DAPI staining. Furthermore, the possible signaling pathway was explored by Western blot. In Capan-1 pancreatic cancer cells, our observations demonstrated that this strategy could enhance potential anticancer efficiency by inducing apoptosis. The mechanisms of the synergistic effect may be due to reducing protein expression of Bcl-2 and enhancing that of Bax, caspase 9, and caspase 3.

CONCLUSION:

These findings demonstrate that a combination of GA and MNPs-Fe(3)O(4) represents a promising approach to the treatment of pancreatic cancer.

• Authors: Cailian Wang, Haijun Zhang, Baoan Chen, Haitao Yin, Wenwen Wang
• Journal: International journal of nanomedicine (Int J Nanomedicine) Vol. 6 Pg. 1929-35 ( 2011) ISSN: 1178-2013 [Electronic] New Zealand
• PMID: 21931488 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• BAX protein, human
• Drug Carriers
• Magnetite Nanoparticles
• Proto-Oncogene Proteins c-bcl-2
• Xanthones
• bcl-2-Associated X Protein
• gambogic acid
• CASP3 protein, human
• CASP9 protein, human
• Caspase 3
• Caspase 9

Topics

• Antineoplastic Agents, Phytogenic (administration & dosage)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Caspase 9 (metabolism)
• Cell Line, Tumor
• Drug Carriers
• Drug Delivery Systems
• Humans
• Magnetite Nanoparticles (administration & dosage, ultrastructure)
• Models, Biological
• Nanomedicine
• Pancreatic Neoplasms (drug therapy, metabolism, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Xanthones (administration & dosage)
• bcl-2-Associated X Protein (metabolism)