Association of matrix metalloproteinase-7 (-181A>G) polymorphism with risk of esophageal squamous cell carcinoma in Kashmir Valley.

Abstract

BACKGROUND/AIM:

Degradation of the basement membrane and extracellular matrix by matrix metalloproteinases (MMPs) is believed to be an essential step in the complicated process of hematogenous metastasis. Matrix metalloproteinase-7 (MMP-7) is a small secreted proteolytic enzyme with a broad substrate specificity, and its expression has been shown to be associated with tumor invasion and metastasis for various cancers.

PATIENTS AND METHODS:

To document the role of MMP-7 polymorphism in esophageal carcinogenesis, a case-control study was performed comprising 135 patients with esophageal cancer (EC) and 195 healthy controls. Genotyping was done by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Data were statistically analyzed using χ2 - test and logistic regression models.

RESULTS:

Carriers for the MMP-7 (-181A>G) GG were associated with an increased risk for EC [odds ratio (OR = 2.17; 95% confidence interval (CI) = 1.21-3.92; P = 0.010; P-trend = 0.04]. Also, in a recessive model, our results showed that MMP-7 (-181A>G) GG allele conferred significantly higher risk for EC (OR =2.16; 95% CI = 1.31-3.54; P = 0.003). The high risk due to MMP-7 (-181GG) genotype was limited to squamous cell histology of EC (OR = 2.41; 95% CI = 1.27-4.56; P = 0.007). Although smoking (Hukka) and high consumption of salted tea are independent risk factors for EC, the interaction of MMP-7 (-181A>G) genotypes with these factors did not further modulate the risk of EC.

CONCLUSIONS:

In conclusion, our results show that MMP-7 (-181A>G) GG carriers are at a higher risk of esophageal squamous cell carcinoma in Kashmir valley.

• Authors: Manzoor A Malik, Kiran L Sharma, Showket A Zargar, Balraj Mittal
• Journal: Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association (Saudi J Gastroenterol) 2011 Sep-Oct Vol. 17 Issue 5 Pg. 301-6 ISSN: 1998-4049 [Electronic] India
• PMID: 21912055 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• MMP7 protein, human
• Matrix Metalloproteinase 7

Topics

• Alleles
• Carcinoma, Squamous Cell (enzymology, epidemiology, genetics)
• DNA, Neoplasm (genetics)
• Esophageal Neoplasms (enzymology, epidemiology, genetics)
• Female
• Follow-Up Studies
• Genetic Predisposition to Disease
• Genotype
• Humans
• Incidence
• India (epidemiology)
• Male
• Matrix Metalloproteinase 7 (genetics, metabolism)
• Middle Aged
• Neoplasm Staging
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Prevalence
• Promoter Regions, Genetic
• Retrospective Studies
• Risk Factors