HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cells.

Abstract
The aim of this study was to explore the effect of bisphenol A (BPA) on the EGFR-STAT3 pathway in breast cancer. We applied 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity assay to the analysis of the responsiveness of MCF-7 cells to BPA. Gene expression was assayed at the transcriptional and translational levels by reverse transcription-PCR and Western blotting. We explored the effects of BPA on MCF-7 cell proliferation through inhibition of the related genes, STAT3, using RNA interference, and EGFR, using its inhibitor AG1478. The optimal concentration and time point of BPA-induced proliferation in MCF-7 cells are 1 µM and 24 h, respectively. BPA significantly increased the expression of STAT3 at a concentration of 1 µM following treatment for 48 h and the expression of STAT3 was down-regulated after blocking EGFR. When STAT3 was blocked in MCF-7 cells, BPA did not appear to induce cell proliferation. Treatment with BPA (1 µM) in the presence of AG1478 for 48 h resulted in the stimulation of cell growth in MCF-7 cells, similar to that of the BPA alone treatment. BPA increases STAT3 expression, which is a major factor in the pathway of BPA-induced proliferation, and STAT3 activation contributes to BPA-induced breast cancer cell proliferation. However, EGFR mediates negative signaling for BPA-induced breast cancer cell proliferation.
AuthorsWei Zhang, Yanqiu Fang, Xu Shi, Minglei Zhang, Xiaoqi Wang, Yan Tan
JournalMolecular medicine reports (Mol Med Rep) Vol. 5 Issue 1 Pg. 41-7 (01 2012) ISSN: 1791-3004 [Electronic] Greece
PMID21909620 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Benzhydryl Compounds
  • Phenols
  • Quinazolines
  • RNA, Small Interfering
  • STAT3 Transcription Factor
  • Tyrphostins
  • RTKI cpd
  • ErbB Receptors
  • bisphenol A
Topics
  • Benzhydryl Compounds
  • Breast Neoplasms
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • ErbB Receptors (antagonists & inhibitors, genetics, metabolism)
  • Female
  • Gene Expression Regulation, Neoplastic (drug effects)
  • Humans
  • Phenols (pharmacology)
  • Quinazolines (pharmacology)
  • RNA Interference
  • RNA, Small Interfering (metabolism)
  • STAT3 Transcription Factor (antagonists & inhibitors, genetics, metabolism)
  • Signal Transduction (drug effects)
  • Time Factors
  • Tyrphostins (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: