Protection against rotavirus challenge in mice orally inoculated with trypsin inhibitor.

Abstract

BACKGROUND AND OBJECTIVES:

the burden of rotavirus disease is significant in developed as well as developing countries and has not diminished with improved hygiene. In the present studies the protective efficacy of soyabean trypsin inhibitor (TI) was investigated on the gut following rotavirus (RV) infection in inbred mice.

METHODS:

infant BALB/c mice of 7 days age (n=108) were divided into three equal groups (control, RV and RV+TI). Animals were orally inoculated either with normal saline, EB RV (serotype-3) or RV+TI and were sacrificed under light chloroform anesthesia on days 0, 1, 3, 5, 7 and 10 post inoculation (pi). Gamma-glutamyl transpeptidase (GGT), leucine aminopeptidase (LAP), and leucine amino acid uptake in the jejunum and ileum were assessed.

RESULTS:

rotavirus inoculated animals showed significant reduction in body weights on days 3 and 5 pi as a result of diarrhoea which resolved by day 7 pi. In RV+TI inoculated animals diarrhoea jejunum and ileum in mice inoculated with RV as compared to controls. With the administration of TI the amino acid uptake, and GGT and LAP levels were higher in both segments of the small intestine and comparable to the controls.

INTERPRETATION AND CONCLUSIONS:

on the administration of TI, leucine amino acid uptake, and levels of GGT and LAP were similar to the controls in the jejunum and ileum of infant mice showing its possible protective efficacy during rotavirus infection.

• Authors: R Katyal, V Singh, S V Rana, S Ojha, K Vaiphie, K Singh
• Journal: The Indian journal of medical research (Indian J Med Res) Vol. 113 Pg. 40-7 (Feb 2001) ISSN: 0971-5916 [Print] India
• PMID: 21901906 (Publication Type: Journal Article)

Chemical References

• Plant Proteins
• Trypsin Inhibitors

Topics

• Administration, Oral
• Animals
• Animals, Suckling
• Diarrhea (drug therapy, virology)
• Female
• Mice
• Mice, Inbred BALB C
• Plant Proteins (pharmacology)
• Rotavirus (drug effects, growth & development)
• Rotavirus Infections (drug therapy, prevention & control)
• Soybeans
• Trypsin Inhibitors (pharmacology)