Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine.

In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval Egyptian ticks, Hyalomma (H.) dromedarii, using a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines against camel tick infestation in rabbits. The rabbits received three intramuscular inoculations of GLPs (20 µg/animal) on days 0, 14, and 28. In the immunoblot analysis, Sera from the immunized rabbits recognized the native GLPs and other proteins from larval and adult H. dromedarii ticks along with those from other tick species such as Rhipicephalus sanguineus but not Ornithodoros moubata. The effects of immunity induced by these GLPs were determined by exposing rabbits to adult H. dromedarii ticks. These results demonstrated that GLP immunization led to a slightly decreased reproductive index and significantly reduced rates of egg hatchability. These results demonstrated that immunization with the purified GLPs can provide protection against infestation by H. dromedarii and some other tick species. Further studies are needed to confirm the effectiveness of immunization with GLPs against other tick species.
AuthorsAmr E El Hakim, Yasser E Shahein, Sobhy Abdel-Shafy, Amira Mk Abouelella, Ragaa R Hamed
JournalJournal of veterinary science (J Vet Sci) Vol. 12 Issue 3 Pg. 243-9 (Sep 2011) ISSN: 1976-555X [Electronic] Korea (South)
PMID21897098 (Publication Type: Journal Article)
Chemical References
  • Antigens
  • Glycoproteins
  • Animals
  • Antigens (immunology, isolation & purification)
  • Argasidae (immunology)
  • Chromatography, Affinity (veterinary)
  • Electrophoresis, Polyacrylamide Gel (veterinary)
  • Female
  • Glycoproteins (immunology, isolation & purification)
  • Immunoblotting (veterinary)
  • Injections, Intramuscular (veterinary)
  • Ixodidae (growth & development, immunology)
  • Life Cycle Stages
  • Male
  • Rabbits (immunology, parasitology)
  • Reproduction
  • Species Specificity
  • Tick Infestations (immunology, prevention & control, veterinary)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: