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Induction of virus-specific neutralizing immune response against West Nile and Japanese encephalitis viruses by chimeric peptides representing T-helper and B-cell epitopes.

Abstract
West Nile virus (WNV) and Japanese encephalitis virus (JEV), the members of JEV serocomplex group are pathogens of global health concern. The co-circulation of these viruses poses challenges in effective diagnostics due to antigenic similarity between the E-protein of these viruses. The present study aimed to design chimeric peptides and study the immune response against the same. B-cell epitopes were predicted on structural proteins of WNV and JEV based on bioinformatics tools. The peptides representing to these B-cell epitopes were synthesized and subjected to ELISA. Two peptides, one each from WNV (named WE147) and JEV (named JE40) E-protein, showed virus-specific and strong reactivity to the immune mice sera and human clinical samples. The chimeric peptides for WNV and JEV were constructed by synthesizing the B-cell epitope of WNV (WE147) or JEV (JE40) with T-helper epitope (JM17) separated by diglycine spacer in between. The immune response generated against these chimeric peptides was found to be specific to the respective B-cell epitopes. The anti-peptide sera showed virus-specific reactivity in ELISA and in immunofluorescence assay with no cross-reactivity. Also, the anti-peptide sera could neutralize JE and WN viruses in an in vitro virus neutralization assay. The B-cell epitopes identified in the present study may be used as diagnostic markers for differentiating between WN and JE virus infections. The present study can form a basis for future design of vaccines.
AuthorsRoopesh Singh Gangwar, Pratip Shil, Gajanan N Sapkal, Siraj A Khan, Milind M Gore
JournalVirus research (Virus Res) Vol. 163 Issue 1 Pg. 40-50 (Jan 2012) ISSN: 1872-7492 [Electronic] Netherlands
PMID21889960 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier B.V. All rights reserved.
Chemical References
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Epitopes, B-Lymphocyte
  • Epitopes, T-Lymphocyte
  • Recombinant Fusion Proteins
Topics
  • Animals
  • Antibodies, Neutralizing (blood)
  • Antibodies, Viral (blood)
  • Encephalitis Virus, Japanese (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes, B-Lymphocyte (genetics, immunology)
  • Epitopes, T-Lymphocyte (genetics, immunology)
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Fusion Proteins (genetics, immunology)
  • West Nile virus (immunology)

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