An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes.

Abstract

Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.

• Authors: Aleah L Brubaker, David F Schneider, Jessica L Palmer, Douglas E Faunce, Elizabeth J Kovacs
• Journal: Journal of immunological methods (J Immunol Methods) Vol. 373 Issue 1-2 Pg. 161-6 (Oct 28 2011) ISSN: 1872-7905 [Electronic] Netherlands
• PMID: 21889511 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Topics

• Animals
• Cell Separation (methods)
• Cells, Cultured
• Flow Cytometry (methods)
• Leukocytes (immunology, pathology)
• Mice
• Phagocytosis (immunology)
• Skin (immunology, injuries, pathology)
• Staphylococcus aureus (immunology)