Abstract |
Aberrant HLA-G expression is associated with tumor invasiveness and poor clinical prognosis; however, there is a lack of preclinical animal model to address whether HLA-G plays a causal role in the unfavorable prognosis of malignancies. In the current study, ovarian carcinoma cell lines (HO-8910 and Ovcar-3) were transfected with HLA-G gene. HLA-G expression was analyzed with western blot and flow cytometry. Transwell experiment was performed to analyze the cell migration and invasion capability and/or multicellular spheroid formation was investigated with the 3D culture assay in vitro. The effects of HLA-G expression for tumor cell organ metastasis and for mouse survival was analyzed with the Balb/c nu/nu mouse model. Our data showed that HO-8910-G and Ovcar-3-G cells are of higher invasion potential compared with the parental HO-8910 and Ovcar-3 cells. Multicellular spheroid formation exists only in HO-8910-G cells in a 3D culture assay. In Balb/c nu/nu mouse model, widespread metastasis was observed in mice xenografted with HO-8910-G cells, but not in the group with parental cells. Mouse survival was dramatically decreased in HO-8910-G and Ovcar-3-G xenografted mice than that with HO-8910 and Ovcar-3 cells, respectively. In summary, our study provided the first evidence that HLA-G expression is associated with tumor metastasis and with poor survival in an animal model with ovarian cancer.
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Authors | Aifen Lin, Xia Zhang, Hui-Hui Xu, Dan-Ping Xu, Yan-Yun Ruan, Wei-Hua Yan |
Journal | International journal of cancer
(Int J Cancer)
Vol. 131
Issue 1
Pg. 150-7
(Jul 01 2012)
ISSN: 1097-0215 [Electronic] United States |
PMID | 21858813
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 UICC. |
Chemical References |
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Topics |
- Animals
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Disease Models, Animal
- Female
- Gene Expression Regulation, Neoplastic
- HLA-G Antigens
(biosynthesis, genetics)
- Humans
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Invasiveness
- Neoplasm Metastasis
(immunology)
- Ovarian Neoplasms
(immunology, mortality, pathology)
- Transfection
(methods)
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