Abstract | BACKGROUND: Dengue virus (DENV) infection is a serious public health issue without specific treatment. We examined the potential immunomodulatory effects of leflunomide, a dihydroorotate dehydrogenase inhibitor commonly prescribed for arthritis, in DENV-stimulated monocyte-derived dendritic cells (mo-DCs). METHODS: mo-DCs were prepared from purified monocytes. Cytokine and chemokine concentrations were determined by enzyme-linked immunosorbent assay. Expression of cell surface markers or viral E protein was measured by flow cytometry. The activation of transcription factors and kinases was determined by electrophoretic mobility shift assays, Western blotting, or immunoprecipitation kinase assays. Chemotaxis assays were used to determine cell migration. RESULTS: CONCLUSIONS:
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Authors | Wan-Lin Wu, Ling-Jun Ho, Pei-Chih Chen, Yi-Ting Tsai, Seng-Ting Hsu, Deh-Ming Chang, Jenn-Haung Lai |
Journal | Journal of clinical immunology
(J Clin Immunol)
Vol. 31
Issue 6
Pg. 1065-78
(Dec 2011)
ISSN: 1573-2592 [Electronic] Netherlands |
PMID | 21845515
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCR7 protein, human
- Chemokine CCL19
- Chemokine CCL21
- Dihydroorotate Dehydrogenase
- Immunosuppressive Agents
- Isoxazoles
- NF-kappa B
- Receptors, CCR7
- Transcription Factor AP-1
- Viral Envelope Proteins
- E protein TH Sman, Dengue virus
- Oxidoreductases Acting on CH-CH Group Donors
- Leflunomide
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Topics |
- Cell Differentiation
(drug effects)
- Cell Movement
(drug effects)
- Cells, Cultured
- Chemokine CCL19
(genetics, metabolism)
- Chemokine CCL21
(genetics, metabolism)
- Dendritic Cells
(drug effects, immunology, metabolism, pathology)
- Dengue
(drug therapy, immunology)
- Dengue Virus
(immunology, pathogenicity)
- Dihydroorotate Dehydrogenase
- Humans
- Immunosuppressive Agents
(pharmacology)
- Isoxazoles
(pharmacology)
- Leflunomide
- Monocytes
(pathology)
- NF-kappa B
(metabolism)
- Oxidoreductases Acting on CH-CH Group Donors
(antagonists & inhibitors)
- Receptors, CCR7
(genetics, metabolism)
- Signal Transduction
(drug effects)
- Transcription Factor AP-1
(metabolism)
- Viral Envelope Proteins
(immunology)
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