Abstract |
The effects of administration of vitamin D₃ and Seocalcitol on MNU-induced carcinogenesis of mammary gland in Sprague-Dawley rats have been investigated. Administration of both substances in a weekly dose of 7 μg/kg caused prolonged latency of mammary gland tumors. The latency of tumors was markedly prolonged for 30-40 days by Seocalcitol. Using PET analysis, reduction in [¹⁸F] 2-fluoro-2-deoxy-d-glucose (FDG) uptake or tumor volume in tumors chemopreventively treated with vitamin D₃ were detected in MNU-induced tumors, vitamin D₃ reduced expression of 25-hydroxylase (25OHase) (p<0.01) and 24-hydroxylase (24OHase) (p<0.01) and Seocalcitol 24OHase. Positive regulation of 25OHase mRNA level after the treatment with vitamin D₃ was observed in liver, while in kidney, vitamin D₃ and Seocalcitol induced expression of 24OHase was significant. Our observations indicate a cross talk between respective pathways of VDR, RARs/RXRs, TRs and ERs in carcinogenesis process.
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Authors | Dana Macejová, Slavomíra Ondková, Lucia Jakubíková, Alžbeta Mlynarčíková, Soňa Scsuková, Ján Liška, Július Brtko |
Journal | Toxicology letters
(Toxicol Lett)
Vol. 207
Issue 1
Pg. 60-72
(Nov 10 2011)
ISSN: 1879-3169 [Electronic] Netherlands |
PMID | 21843606
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- RNA, Messenger
- Receptors, Calcitriol
- Cholecalciferol
- Methylnitrosourea
- Mixed Function Oxygenases
- Calcitriol
- seocalcitol
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Topics |
- Animals
- Calcitriol
(analogs & derivatives, pharmacology)
- Cell Transformation, Neoplastic
(chemically induced, drug effects)
- Cholecalciferol
(pharmacology)
- Female
- Histocytochemistry
- Mammary Neoplasms, Experimental
(chemically induced, enzymology, metabolism, prevention & control)
- Methylnitrosourea
(toxicity)
- Mixed Function Oxygenases
(genetics, metabolism)
- Positron-Emission Tomography
- RNA, Messenger
(chemistry, genetics)
- Rats
- Rats, Sprague-Dawley
- Receptors, Calcitriol
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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