Abstract |
HMBOX1 is a new member of homeobox family and predicted to be a transcriptional repressor, its function in NK cells is completely unclear. Previously we found that overexpression of HMBOX1 downregulated mRNA level of IFN-γ in NK cells during our gene screening work. In present study, we investigate the relationship between HMBOX1 and IFN-γ in detail. Firstly, we describe the properties of HMBOX1 gene transcription in activated NK cells, and found that the transcriptional levels of HMBOX1 were significantly decreased in NK cells after activated by IL-2, IL-15 and IL-12, which was opposite to the expression profile of IFN-γ. Subsequently, over-expression of HMBOX1 significantly inhibited the expression and production of IFN-γ in NK cells in response to the stimulation of tumor cell K562 or PMA/ ionomycin. Additionally, by luciferase reporter assay, HMBOX1 displayed suppressive effect on the transcription activity of IFN-γ promoter. These findings indicated that HMBOX1 may function as a negative regulator of IFN-γ in NK cells.
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Authors | Longyan Wu, Cai Zhang, Xiaodong Zheng, Zhigang Tian, Jian Zhang |
Journal | International immunopharmacology
(Int Immunopharmacol)
Vol. 11
Issue 11
Pg. 1895-900
(Nov 2011)
ISSN: 1878-1705 [Electronic] Netherlands |
PMID | 21839858
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
Chemical References |
- Cytokines
- HMBOX1 protein, human
- Homeodomain Proteins
- Interferon-gamma
- Luciferases
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Topics |
- Cytokines
(immunology)
- Down-Regulation
- Flow Cytometry
- HEK293 Cells
- Homeodomain Proteins
(genetics, physiology)
- Humans
- Interferon-gamma
(genetics)
- K562 Cells
- Killer Cells, Natural
(immunology, metabolism)
- Luciferases
(genetics)
- Lymphocyte Activation
(immunology)
- Promoter Regions, Genetic
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic
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