Abstract | BACKGROUND/AIMS: METHODOLOGY: Eukaryotic expression plasmids with/without the FHIT gene were constructed and transfected into three groups in which cholangiocarcinoma cells were also divided. We observed the proliferation of cholangiocarcinoma by using the MTT method and flow cyclometry (FCM). Invasive ability was then determined via transwell chamber testing. In addition, to examine whether FHIT mRNA and protein expression were correlated with cyclin D1 expression, we used real-time RT-PCR and western blotting. RESULTS: The absorbance (A490) was decreased significantly (p<0.05) according to MTT, showing that proliferation had been inhibited and the apoptosis ratio after transfecting had increased significantly (p<0.05). The amount of cells which passed the filter in the transwell chamber was significantly less (p<0.05). The expression of cyclin D1 mRNA and the protein was decreased from the original cell line (p<0.05). CONCLUSIONS: The results suggest that increased FHIT gene activity can promote apoptosis of cholangiocarcinoma and reduce the expression level of cyclin D1.
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Authors | Qiang Huang, Fang Xie, Zhigang Tang, Chenhai Liu, Cheng Wang, Yuanguo Hu, Lujun Qiu, Shijie Wang, Hao You, Kai Zhu, Peng Xu |
Journal | Hepato-gastroenterology
(Hepatogastroenterology)
2011 May-Jun
Vol. 58
Issue 107-108
Pg. 713-8
ISSN: 0172-6390 [Print] Greece |
PMID | 21830375
(Publication Type: Journal Article)
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Chemical References |
- CCND1 protein, human
- Neoplasm Proteins
- RNA, Messenger
- fragile histidine triad protein
- Cyclin D1
- Acid Anhydride Hydrolases
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Topics |
- Acid Anhydride Hydrolases
(genetics, physiology)
- Apoptosis
- Bile Duct Neoplasms
(pathology)
- Bile Ducts, Intrahepatic
- Cell Line, Tumor
- Cholangiocarcinoma
(pathology)
- Cyclin D1
(genetics, physiology)
- Humans
- Neoplasm Proteins
(genetics, physiology)
- RNA, Messenger
(analysis)
- Transfection
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