Abstract |
Anti-tumour effector cells were generated through 4 days culture of normal C57BL/6 splenocytes in a medium containing concanavalin A supernatant and then fractionated with Dolichos biflorus lectin (DBA) into DBA+ (agglutinable with DBA) and DBA- (non-agglutinable with DBA) cells. The DBA- cells, infused intravenously into mice together with B16 melanoma cells, or adoptively transferred into mice 3 days after the injection of B16 cells, caused a marked decrease in the number of lung nodules, while the DBA+ cells exerted no effect. On the other hand, the DBA+ cells exhibited higher cytolytic activity in vitro than the DBA- cells in short-term 51Cr-release assays. Then, we analysed the mechanism of the strong anti-tumour activity of DBA- cells in vivo. We found that DBA- cells showed higher response to recombinant interleukin-2 (rIL-2) than DBA+ cells and proliferated very well with a small amount of IL-2. In addition, DBA- cells adhered more strongly to lung endothelial cells than DBA+ cells in response to rIL-1 or rTNF. Furthermore, DBA- cells produced larger amounts of macrophage activating factor (MAF) including IFN-gamma when cultured with B16 melanoma. Taken together, our results show that DBA- cells are effective in reducing experimental pulmonary metastases not only by the direct lytic activity but also by the indirect killing activity through the activated macrophage.
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Authors | T Okada, M Higuchi, M Takano, T Maruyama, Y Imai, T Osawa |
Journal | British journal of cancer
(Br J Cancer)
Vol. 61
Issue 2
Pg. 241-9
(Feb 1990)
ISSN: 0007-0920 [Print] England |
PMID | 2178666
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-2
- Lectins
- Lymphokines
- Macrophage-Activating Factors
- Plant Lectins
- dolichos biflorus agglutinin
- Concanavalin A
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Topics |
- Animals
- Cell Adhesion
(drug effects)
- Cell Separation
- Concanavalin A
(metabolism)
- Endothelium, Vascular
- Female
- Immunization, Passive
- Interleukin-2
(pharmacology)
- Killer Cells, Lymphokine-Activated
(transplantation)
- Lectins
(immunology)
- Lung Neoplasms
(prevention & control, secondary)
- Lymphokines
(analysis)
- Macrophage-Activating Factors
- Melanoma
(prevention & control, secondary)
- Mice
- Mice, Inbred Strains
- Plant Lectins
- Spleen
(transplantation)
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