The activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in cervical cancer.

Abstract

OBJECTIVE:

The aim of this study was to compare the metabolism of cervical cancer cells and normal cervical cells by measurement of ADH isoenzymes and ALDH activities.

METHODS:

The study material consisted of 40 cancerous cervical tissues. Class III, IV of ADH and total ADH activity was measured by the photometric method and class I, II ADH and ALDH activity by the fluorometric method with class-specific fluorogenic substrates.

RESULTS:

The activity of the class I ADH isoenzyme and the total ADH were significantly higher in cervical cancer as compared to healthy tissues. Class I of ADH and total ADH activity are significantly higher in every stage of cancer as compared to the control. There are no significant differences between planoepitheliale and adenocarcinoma.

CONCLUSION:

The increased activity of total ADH in cervical cancer may be the cause of some metabolic disorders in cancer cells, which may intensify carcinogenesis in this organ.

• Authors: Karolina Orywal, Wojciech Jelski, Michał Zdrodowski, Maciej Szmitkowski
• Journal: Clinical biochemistry (Clin Biochem) Vol. 44 Issue 14-15 Pg. 1231-4 (Oct 2011) ISSN: 1873-2933 [Electronic] United States
• PMID: 21784063 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Isoenzymes
• Alcohol Dehydrogenase
• alcohol dehydrogenase IV
• formaldehyde dehydrogenase (glutathione)
• Aldehyde Oxidoreductases
• Aldehyde Dehydrogenase

Topics

• Adenocarcinoma (enzymology)
• Adult
• Aged
• Alcohol Dehydrogenase (metabolism)
• Aldehyde Dehydrogenase (metabolism)
• Aldehyde Oxidoreductases (metabolism)
• Female
• Humans
• Isoenzymes (metabolism)
• Middle Aged
• Neoplasm Staging
• Reference Values
• Uterine Cervical Neoplasms (enzymology, pathology)
• Young Adult