Effects of PerClot® on the healing of full-thickness skin wounds in rats.

Abstract

PerClot(®) is a hemostatic material made of polysaccharide from modified starch and has been shown to assist in topical hemostasis. The principal goal in treating surgical and non-surgical wounds is the need for rapid closure of the lesion. This study investigated whether topical application of PerClot(®) could improve impaired wound healing in Sprague-Dawley (SD) rats. Full-thickness skin wounds were created on the back of the rats. Immediately, PerClot(®) was introduced into the wound bed, while wounds receiving starch or nothing served as controls. Wound closure was monitored using well-recognized wound-healing parameters: histological examination for inflammatory cells and fibroblast infiltration, newly formed capillaries, and collagen deposition. Meanwhile, transforming growth factor (TGF-β1) was measured by immunochemistry. Wound closure was significantly accelerated by local application of PerClot(®). Furthermore, PerClot(®)-treated wounds showed significantly increased fibroblast numbers at 5 days post-wounding, and newly formed capillaries at 7 days post-wounding, and collagen regeneration at 7 and 14 days post-wounding. The number of infiltrating fibroblasts expressing TGF-β1 was significantly higher than that in the controls at 7 and 14 days post-wounding. PerClot(®) can improve the wound healing and this effect might involve an increase in the activity of fibroblasts and increased release of TGF-β1.

• Authors: Yanxia Wang, Min Xu, Haiyin Dong, Yi Liu, Pengtao Zhao, Wen Niu, Dunquan Xu, Xin Ji, Chen Xing, Dongding Lu, Zhichao Li
• Journal: Acta histochemica (Acta Histochem) Vol. 114 Issue 4 Pg. 311-7 (Jul 2012) ISSN: 1618-0372 [Electronic] Germany
• PMID: 21782216 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright © 2011. Published by Elsevier GmbH. All rights reserved.

Chemical References

• Hemostatics
• Transforming Growth Factor beta1
• Collagen

Topics

• Administration, Topical
• Animals
• Capillaries (physiology)
• Cell Count
• Cell Movement (drug effects)
• Collagen (biosynthesis)
• Fibroblasts (cytology, drug effects, metabolism)
• Hemostasis (drug effects, physiology)
• Hemostatics (administration & dosage, therapeutic use)
• Male
• Neovascularization, Physiologic
• Rats
• Rats, Sprague-Dawley
• Skin (blood supply, drug effects, injuries)
• Transforming Growth Factor beta1 (biosynthesis)
• Wound Healing (drug effects, physiology)
• Wounds, Penetrating (drug therapy, pathology)