Kainate postconditioning restores LTP in ischemic hippocampal CA1: onset-dependent second pathophysiological stress.

Abstract

Postconditioning can be induced by a broad range of stimuli within minutes to days after an ischemic cerebral insult. A special form is elicited by pharmacological intervention called second pathophysiological stress. The present study aimed to evaluate the effects of low-dose (5 mg/kg) kainate postconditioning with onsets 0, 24 and 48 h after the ischemic insult on the hippocampal synaptic plasticity in a 2-vessel occlusion model in rat. The hippocampal function was tested by LTP measurements of Schaffer collateral-CA1 pyramidal cell synapses in acute slices and the changes in density of Golgi-Cox-stained apical dendritic spines. Postconditioning 0 and 24 h after ischemia was not protective, whereas 48-h-onset postconditioning resulted in the reappearance of a normal spine density (>100,000 spines) 3 days after ischemia, in parallel with the long-term restoration of the damaged LTP function. Similar, but somewhat less effects were observed after 10 days. Our data clearly demonstrate the onset dependence of postconditioning elicited by a subconvulsant dose of kainate treatment in global ischemia, with restoration of the structural plasticity and hippocampal function.

• Authors: Dávid Nagy, Kitti Kocsis, János Fuzik, Máté Marosi, Zsolt Kis, Vivian I Teichberg, József Toldi, Tamás Farkas
• Journal: Neuropharmacology (Neuropharmacology) 2011 Oct-Nov Vol. 61 Issue 5-6 Pg. 1026-32 ISSN: 1873-7064 [Electronic] England
• PMID: 21781978 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Excitatory Amino Acid Agonists
• Kainic Acid

Topics

• Animals
• CA1 Region, Hippocampal (drug effects, physiology)
• Drug Evaluation, Preclinical
• Electroencephalography
• Electrophysiological Phenomena (drug effects, physiology)
• Excitatory Amino Acid Agonists (pharmacology)
• Ischemic Attack, Transient (mortality, physiopathology)
• Kainic Acid (pharmacology)
• Long-Term Potentiation (drug effects, physiology)
• Male
• Molecular Targeted Therapy
• Pyramidal Cells (drug effects, physiology)
• Random Allocation
• Rats
• Rats, Wistar
• Stress, Physiological (drug effects, physiology)