Abstract |
In the present study, we have investigated the anatomic distribution in blood and gut mucosal tissues of memory poxvirus-specific CD4 and CD8 T cells in subjects vaccinated with smallpox and compared it with vector (NYVAC)-specific and HIV insert-specific T-cell responses induced by an experimental DNA-C/ NYVAC-C vaccine regimen. Smallpox-specific CD4 T-cell responses were present in the blood of 52% of the subjects studied, while smallpox-specific CD8 T cells were rarely detected (12%). With one exception, smallpox-specific T cells were not measurable in gut tissues. Interestingly, NYVAC vector-specific and HIV-specific CD4 and CD8 T-cell responses were detected in almost 100% of the subjects immunized with DNA-C/NYVAC-C in blood and gut tissues. The large majority (83%) of NYVAC-specific CD4 T cells expressed α4β7 integrins and the HIV coreceptor CCR5. These results demonstrate that the experimental DNA-C/NYVAC-C HIV vaccine regimen induces the homing of potentially protective HIV-specific CD4 and CD8 T cells in the gut, the port of entry of HIV and one of the major sites for HIV spreading and the depletion of CD4 T cells.
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Authors | Matthieu Perreau, Hugh C Welles, Alexandre Harari, Olivia Hall, Ricardo Martin, Michel Maillard, Gian Dorta, Pierre-Alexandre Bart, Eric J Kremer, Jim Tartaglia, Ralf Wagner, Mariano Esteban, Yves Levy, Giuseppe Pantaleo |
Journal | Journal of virology
(J Virol)
Vol. 85
Issue 19
Pg. 9854-62
(Oct 2011)
ISSN: 1098-5514 [Electronic] United States |
PMID | 21775454
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AIDS Vaccines
- NYVAC vaccine
- Smallpox Vaccine
- Vaccines, DNA
- Viral Vaccines
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Topics |
- AIDS Vaccines
(administration & dosage, immunology)
- Adult
- Blood
(immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Humans
- Intestinal Mucosa
(immunology)
- Middle Aged
- Smallpox Vaccine
(administration & dosage, immunology)
- Vaccination
(methods)
- Vaccines, DNA
(administration & dosage, immunology)
- Viral Vaccines
(administration & dosage, immunology)
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