Abstract | BACKGROUND: Recently zebrafish has become a powerful vertebrate model system for cardiac arrhythmia and another advantage is that the heart could be rapidly excised for in-vitro electrophysiological recording. METHODS: We made direct in-vitro recordings of adult zebrafish heart ECG using the microelectrode and tested the effects of various drugs on zebrafish heart ECG. RESULTS: CONCLUSIONS: Direct in-vitro recording of adult zebrafish heart is an efficient platform for test of drugs which exert electrophysiological effects on cardiomyocytes, and perturbation of ionic channels that are responsible for human long QT syndrome also modulates QT interval in adult zebrafish heart.
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Authors | Chia-Ti Tsai, Cho-Kai Wu, Fu-Tien Chiang, Chuen-Den Tseng, Jen-Kuang Lee, Chih-Chieh Yu, Yi-Chih Wang, Ling-Ping Lai, Jiunn-Lee Lin, Juey-Jen Hwang |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 412
Issue 21-22
Pg. 1963-7
(Oct 09 2011)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 21767531
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
Chemical References |
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
- Quinidine
- Isoproterenol
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Topics |
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
(pharmacology)
- Animals
- Dose-Response Relationship, Drug
- Electrocardiography
- Heart Rate
(drug effects)
- Isoproterenol
(pharmacology)
- Quinidine
(pharmacology)
- Zebrafish
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