Dose-dependent protective effect of sildenafil citrate on testicular injury after torsion/detorsion in rats.

Abstract

This experiment was designed to investigate the effect of sildenafil citrate on testicular injury after unilateral testicular torsion/detorsion (T/D). Thirty-seven adult male Wistar albino rats were divided into four groups: sham operated group (group 1), T/D+ saline (group 2), T/D+ 0.7 mg sildenafil citrate (group 3) and T/D+ 1.4 mg sildenafil citrate (group 4). Testicular torsion was created by rotating the right testis 720° in a clockwise direction for 2 h in other groups, except for group 1, which was served as sham group. The level of GSH (P < 0.05) in the testis in the group 2 were significantly lower (P < 0.05) and the levels of MDA and NO (P < 0.01 for both) in the testis were significantly higher when compared with those of the group 1. Administration of low dose sildenafil citrate prevented the increases in MDA and NO levels and decreases in GSH values induced by testicular torsion. However, administration of high dose sildenafil citrate did not have any effect on these testicular tissue parameters (P > 0.05). Also, mean values of seminiferous tubules diameters, germinal cell layer thicknesses and mean testicular biopsy score were significantly better in group 3 than groups 2 and 4. These results suggest that T/D injury occurred in testis after unilateral testicular T/D and that administration of low dose sildenafil citrate before detorsion prevents ischemia/reperfusion cellular damage in testicular torsion. Sildenafil citrate probably acts through reduction of reactive oxygen species and support antioxidant enzyme systems.

• Authors: H Yıldız, A S Durmus, H Şimşek, M Yaman
• Journal: Andrologia (Andrologia) Vol. 44 Suppl 1 Pg. 300-6 (May 2012) ISSN: 1439-0272 [Electronic] Germany
• PMID: 21749432 (Publication Type: Journal Article)
• Copyright: © 2011 Blackwell Verlag GmbH.

Chemical References

• Phosphodiesterase Inhibitors
• Piperazines
• Purines
• Sulfones
• Nitric Oxide
• Malondialdehyde
• Sildenafil Citrate
• Glutathione

Topics

• Animals
• Dose-Response Relationship, Drug
• Glutathione (metabolism)
• Lipid Peroxidation
• Male
• Malondialdehyde (metabolism)
• Nitric Oxide (metabolism)
• Phosphodiesterase Inhibitors (therapeutic use)
• Piperazines (therapeutic use)
• Purines (therapeutic use)
• Rats
• Rats, Wistar
• Sildenafil Citrate
• Spermatic Cord Torsion (complications)
• Sulfones (therapeutic use)
• Testis (injuries, pathology)