Abstract |
In the present study, the effect of chromaffin cell transplant in the spinal cord was evaluated on formalin-induced mechanical secondary allodynia in the rat. Chromaffin cells were transplanted into the lumbar subarachnoid space before or after formalin injection. Subcutaneous formalin injection (50 μl, 1%) produced long-lasting secondary allodynia in the ipsilateral and contralateral hind paws. Once secondary allodynia was established, treatment with chromaffin cells produced a significant reduction in the nociceptive behavior in both hind paws. The antiallodynic effect was time-dependent since it was observed 15 days after chromaffin cell transplants but not before. On the other hand, pre-treatment with chromaffin cells prevented the expression of secondary allodynia in both hind paws in the rat. Antiallodynic effect of chromaffin cells was reverted with the non-selective opioid receptor antagonist naltrexone and the non-selective α(2)-adrenoceptor antagonist rauwolscine. Clusters of viable chromaffin cells labeled with anti- tyrosine hydroxylase antibodies were observed in the retrieved transplants 15 days after transplant. These results establish the analgesic efficacy of intrathecal chromaffin cells on formalin-induced secondary allodynia. Our data suggest that chromaffin cells release neuroactive substances including opioid peptides and adrenergic amines that reduce secondary allodynia in rats through activation of their receptors.
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Authors | Mónica Ambriz-Tututi, Violeta Sánchez-González, René Drucker-Colín |
Journal | European journal of pharmacology
(Eur J Pharmacol)
Vol. 668
Issue 1-2
Pg. 147-54
(Oct 01 2011)
ISSN: 1879-0712 [Electronic] Netherlands |
PMID | 21741968
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
Chemical References |
- Adrenergic alpha-2 Receptor Antagonists
- Narcotic Antagonists
- Receptors, Adrenergic, alpha-2
- Receptors, Opioid
- Formaldehyde
- Yohimbine
- Naltrexone
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Topics |
- Adrenergic alpha-2 Receptor Antagonists
(pharmacology)
- Animals
- Cell Survival
(drug effects)
- Chromaffin Cells
(transplantation)
- Female
- Formaldehyde
(adverse effects)
- Hyperalgesia
(chemically induced, metabolism, pathology, surgery)
- Naltrexone
(pharmacology)
- Narcotic Antagonists
- Rats
- Rats, Wistar
- Receptors, Adrenergic, alpha-2
(metabolism)
- Receptors, Opioid
(metabolism)
- Spinal Cord
(drug effects, metabolism, pathology)
- Yohimbine
(pharmacology)
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