Abstract | BACKGROUND: METHODOLOGY AND PRINCIPAL FINDINGS: Four serine protease-encoding genes from the venom gland transcriptome of Bitis gabonica rhinoceros were amplified and sequenced. Mass spectrometry suggests the four enzymes corresponding to these genes are present in the venom of B. g. rhinoceros. Two of the enzymes, rhinocerases 2 and 3 have substitutions to two of the serine protease catalytic triad residues and are thus unlikely to be catalytically active, though they may have evolved other toxic functions. The other two enzymes, rhinocerases 4 and 5, have classical serine protease catalytic triad residues and thus are likely to be catalytically active, however they have glycine rather than the more typical aspartic acid at the base of the primary specificity pocket (position 189). Based on a detailed analysis of these sequences we suggest that alternative splicing together with individual amino acid mutations may have been involved in their evolution. Changes within amino acid segments which were previously proposed to undergo accelerated change in venom serine proteases have also been observed. CONCLUSIONS AND SIGNIFICANCE: Our study provides further insight into the diversity of serine protease isoforms present within snake venom and discusses their possible functions and how they may have evolved. These multiple serine protease isoforms with different substrate specificities may enhance the envenomation effects and help the snake to adapt to new habitats and diets. Our findings have potential for helping the future development of improved therapeutics for snake bites.
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Authors | Sakthivel Vaiyapuri, Simon C Wagstaff, Robert A Harrison, Jonathan M Gibbins, E Gail Hutchinson |
Journal | PloS one
(PLoS One)
Vol. 6
Issue 6
Pg. e21532
( 2011)
ISSN: 1932-6203 [Electronic] United States |
PMID | 21731776
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Viper Venoms
- Serine Endopeptidases
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Topics |
- Amino Acid Sequence
- Animal Structures
(enzymology)
- Animals
- Evolution, Molecular
- Gene Expression Profiling
- Models, Molecular
- Molecular Sequence Data
- Phylogeny
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Analysis, Protein
- Sequence Homology, Amino Acid
- Serine Endopeptidases
(chemistry, genetics, metabolism)
- Viper Venoms
(enzymology, genetics)
- Viperidae
(genetics)
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