HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Immune correlates of protection against yellow fever determined by passive immunization and challenge in the hamster model.

Abstract
Live, attenuated yellow fever (YF) 17D vaccine is highly efficacious but causes rare, serious adverse events resulting from active replication in the host and direct viral injury to vital organs. We recently reported development of a potentially safer β-propiolactone-inactivated whole virion YF vaccine (XRX-001), which was highly immunogenic in mice, hamsters, monkeys, and humans [10,11]. To characterize the protective efficacy of neutralizing antibodies stimulated by the inactivated vaccine, graded doses of serum from hamsters immunized with inactivated XRX-001 or live 17D vaccine were transferred to hamsters by the intraperitoneal (IP) route 24h prior to virulent, viscerotropic YF virus challenge. Neutralizing antibody (PRNT(50)) titers were determined in the sera of treated animals 4h before challenge and 4 and 21 days after challenge. Neutralizing antibodies were shown to mediate protection. Animals having 50% plaque reduction neutralization test (PRNT(50)) titers of ≥40 4h before challenge were completely protected from disease as evidenced by viremia, liver enzyme elevation, and protection against illness (weight change) and death. Passive titers of 10-20 were partially protective. Immunization with the XRX-001 vaccine stimulated YF neutralizing antibodies that were equally effective (based on dose response) as antibodies stimulated by live 17D vaccine. The results will be useful in defining the level of seroprotection in clinical studies of new yellow fever vaccines.
AuthorsJustin G Julander, Dennis W Trent, Thomas P Monath
JournalVaccine (Vaccine) Vol. 29 Issue 35 Pg. 6008-16 (Aug 11 2011) ISSN: 1873-2518 [Electronic] Netherlands
PMID21718741 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
CopyrightCopyright © 2011 Elsevier Ltd. All rights reserved.
Chemical References
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Vaccines, Inactivated
  • Yellow Fever Vaccine
Topics
  • Animals
  • Antibodies, Neutralizing (blood, immunology)
  • Antibodies, Viral (blood, immunology)
  • Cricetinae
  • Disease Models, Animal
  • Female
  • Humans
  • Immunization, Passive
  • Mesocricetus
  • Survival Analysis
  • Vaccines, Inactivated (administration & dosage, immunology)
  • Yellow Fever (immunology, mortality, prevention & control)
  • Yellow Fever Vaccine (administration & dosage, immunology)
  • Yellow fever virus (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: