Abstract | INTRODUCTION: TP53 is a tumor suppressor gene located on chromosome 17p13.1. This gene is essential for the control of cell cycle and has been found altered in about 50% of all tumor types. OBJECTIVE: The presence of aneuploidy of chromosome 17 and TP53 gene deletion at 17p13.1 locus was determined in primary solid tumors using the dual-color FISH (fluorescence in situ hybridization). MATERIALS AND METHODS: Thirty-eight samples consisted of several types of primary solid tumors. All samples were mechanically and enzymatically disaggregated with 0.2% collagenase prior to obtaining interphase nuclei. The dual-color FISH was performed using direct fluorescent labeling probes for the chromosome 17 centromere (green signal) and for the TP53 gene locus-specific (orange signal). RESULTS: Characteristic aneuploidy on chromosome 17 was found in 63% (24/38) of the samples. Monosomy occurred most frequently (75%, 18/24), followed by trisomy (17%, 4/24); nullisomy and tetrasomy were less frequent. TP53 gene deletion was found in 89.5% (34/38) of cases. Only four tumors were normal for copy number of chromosome 17 and TP53 gene. The histopathologic study showed that most of the samples were malignant tumors. CONCLUSIONS: Aneuploidy of chromosome 17 and deletion at 17p13.1 locus of TP53 gene were genetic alterations found to be very frequent in solid tumors. The dual-color FISH was able to detect both numerical and structural chromosomal abnormalities in interphase nuclei.
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Authors | Juan Carlos Herrera, Luis Fernando Isaza, José Luis Ramírez, Gonzalo Vásquez, Carlos Mario Muñetón |
Journal | Biomedica : revista del Instituto Nacional de Salud
(Biomedica)
Vol. 30
Issue 3
Pg. 390-400
( 2010)
ISSN: 2590-7379 [Electronic] Colombia |
Vernacular Title | Detección de aneuploidías del cromosoma 17 y deleción del gen TP53 en una amplia variedad de tumores sólidos mediante hibridación in situ fluorescente bicolor. |
PMID | 21713341
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adult
- Aged
- Aneuploidy
- Cell Nucleus
(genetics)
- Chromosome Aberrations
- Chromosomes, Human, Pair 17
(genetics)
- Colombia
- Female
- Gene Deletion
- Genes, p53
- Humans
- In Situ Hybridization, Fluorescence
(methods)
- Male
- Middle Aged
- Neoplasms
(genetics)
- Prospective Studies
- Young Adult
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