Different mechanisms of Ca2+ entry induced by depolarization and muscarinic receptor stimulation in SH-SY5Y human neuroblastoma cells.

Abstract

Depolarization by elevated K+ and stimulation of muscarinic M3 receptors evoke rises in [Ca2+]i in Fura 2-loaded SH-SY5Y human neuroblastoma cells. The response to K+ (30 and 60 mM) could be inhibited by the dihydropyridine L-channel antagonist +PN 200-110 and totally suppressed by Ni2+, the N-channel blocker omega-conotoxin reduced the response to 60 mM K+. Carbachol-stimulated increase in [Ca2+]i was blocked by atropine and Ni2+ but was totally resistant to the L- and N-channel blockers. This study reveals the presence of L- and N-type voltage-sensitive Ca2+ channels on undifferentiated SH-SY5Y cells that are opened by K+ depolarization but not by muscarinic stimulation.

• Authors: D G Lambert, E M Whitham, J G Baird, S R Nahorski
• Journal: Brain research. Molecular brain research (Brain Res Mol Brain Res) Vol. 8 Issue 3 Pg. 263-6 (Aug 1990) ISSN: 0169-328X [Print] Netherlands
• PMID: 2170805 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium Channel Blockers
• Oxadiazoles
• Receptors, Muscarinic
• Atropine
• Nickel
• Carbachol
• Potassium
• Calcium
• Isradipine

Topics

• Atropine (pharmacology)
• Calcium (metabolism)
• Calcium Channel Blockers (pharmacology)
• Carbachol (pharmacology)
• Cell Line
• Cell Membrane (drug effects, physiology)
• Humans
• Isradipine
• Kinetics
• Membrane Potentials (drug effects)
• Neuroblastoma
• Nickel (pharmacology)
• Oxadiazoles (pharmacology)
• Potassium (pharmacology)
• Receptors, Muscarinic (drug effects, physiology)