In vitro effect of calcium dobesilate on oxidative/inflammatory stress in human varicose veins.

Abstract

AIM:

To determine whether calcium dobesilate can act in chronic venous insufficiency by similar antioxidant, anti-inflammatory mechanisms as in diabetic retinopathy.

METHODS:

Calcium dobesilate was tested in vitro for its protective action against oxidative/inflammatory stress in human varicose veins. Varicose greater saphenous veins were obtained from 14 patients (11 men, 3 women) aged 53-65 years. Oxidative stress was induced exogenously in the vein segments, with the phenazine methosulphate (PMS)/NADH couple. Total antioxidant status (TAS) and malondialdehyde (MDA) contents were used as markers of oxidative stress.

RESULTS:

Calcium dobesilate significantly prevented oxidative disturbances in the micromolar range. PMS/NADH-dependent TAS decrease was fully prevented with IC(50) = 11.4 ± 2.3 µmol/L (n = 6 veins), whereas MDA increase was fully prevented with IC(50) = (102 ± -3) µmol/L (n = 6 veins). Calcium dobesilate acted quali- and quantitatively like rutin, the reference compound. Comparison with pharmacokinetic data suggests that calcium dobesilate can act at therapeutic concentrations.

CONCLUSION:

Calcium dobesilate protected human varicose veins against oxidative stress in vitro at levels that correspond to therapeutic concentrations. Further studies are required to investigate whether a similar action is found in varicose veins from patients orally treated with calcium dobesilate.

• Authors: O Alda, M S Valero, D Pereboom, P Serrano, J M Azcona, R P Garay
• Journal: Phlebology (Phlebology) Vol. 26 Issue 8 Pg. 332-7 (Dec 2011) ISSN: 1758-1125 [Electronic] England
• PMID: 21705478 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Hemostatics
• Methylphenazonium Methosulfate
• Malondialdehyde
• Calcium Dobesilate

Topics

• Aged
• Antioxidants (metabolism)
• Calcium Dobesilate (pharmacology)
• Dose-Response Relationship, Drug
• Female
• Hemostatics (pharmacology)
• Humans
• Inflammation (chemically induced, drug therapy, pathology, physiopathology)
• Male
• Malondialdehyde (metabolism)
• Methylphenazonium Methosulfate (toxicity)
• Middle Aged
• Organ Culture Techniques
• Oxidative Stress (drug effects)
• Saphenous Vein (metabolism, pathology, physiopathology)
• Varicose Veins (drug therapy, metabolism, pathology, physiopathology)