Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus and associated toxin genes.

Abstract

We describe a real-time PCR assay for the detection of methicillin-resistant Staphylococcus aureus and genes encoding toxic shock syndrome toxin 1 and Panton-Valentine leukocidin. Rapid screening and detection of toxins is a useful tool for surveillance studies and outbreak investigations involving large numbers of isolates.

• Authors: G E Fosheim, A C Nicholson, V S Albrecht, B M Limbago
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 49 Issue 8 Pg. 3071-3 (Aug 2011) ISSN: 1098-660X [Electronic] United States
• PMID: 21697325 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Bacterial Toxins
• DNA, Bacterial
• Enterotoxins
• Exotoxins
• Leukocidins
• Panton-Valentine leukocidin
• Superantigens
• enterotoxin F, Staphylococcal

Topics

• Bacterial Toxins (analysis, genetics)
• Bacteriological Techniques (methods)
• DNA, Bacterial (genetics)
• Enterotoxins (analysis, genetics)
• Exotoxins (analysis, genetics)
• Genes, Bacterial
• Humans
• Leukocidins (analysis, genetics)
• Mass Screening (methods)
• Methicillin-Resistant Staphylococcus aureus (genetics, isolation & purification)
• Multiplex Polymerase Chain Reaction (methods)
• Real-Time Polymerase Chain Reaction (methods)
• Staphylococcal Infections (microbiology)
• Superantigens (analysis, genetics)