Abstract | BACKGROUND: METHODS: RESULTS: Expression of EpCAM was correlated with DNA methylation and histone modifications. Treatment with 5-AZAC induced EpCAM expression in negative cells. Ten transcription factors were associated with the epcam gene in EpCAM expressing cells, but not in EpCAM-negative cells. Methylation of an Sp1 probe inhibited the binding of nuclear extract proteins in electromobility shift assays; such DNA methylation sensitivity was not observed for an NF-κB probe. CONCLUSION: This study provides insights in transcriptional regulation of epcam in ovarian cancer. Epigenetic parameters associated with EpCAM overexpression are potentially reversible, allowing novel strategies for sustained silencing of EpCAM expression.
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Authors | B T F van der Gun, M L de Groote, H G Kazemier, A J Arendzen, P Terpstra, M H J Ruiters, P M J McLaughlin, M G Rots |
Journal | British journal of cancer
(Br J Cancer)
Vol. 105
Issue 2
Pg. 312-9
(Jul 12 2011)
ISSN: 1532-1827 [Electronic] England |
PMID | 21694727
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- Biomarkers, Tumor
- Cell Adhesion Molecules
- EPCAM protein, human
- Epithelial Cell Adhesion Molecule
- Genetic Markers
- Transcription Factors
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Topics |
- Antigens, Neoplasm
(genetics, metabolism)
- Base Sequence
- Biomarkers, Tumor
(genetics, metabolism)
- Carcinoma
(genetics, metabolism)
- Cell Adhesion Molecules
(genetics, metabolism)
- Cell Line, Tumor
- DNA Methylation
(physiology)
- Epigenesis, Genetic
(physiology)
- Epithelial Cell Adhesion Molecule
- Female
- Gene Expression Regulation, Neoplastic
- Genetic Markers
(physiology)
- Humans
- Models, Biological
- Molecular Sequence Data
- Ovarian Neoplasms
(genetics, metabolism)
- Transcription Factors
(genetics, metabolism, physiology)
- Up-Regulation
(genetics)
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