[Treatment of acute liver injury by intrasplenic transplantation of hepatic stem cells combined with heparin in rats].

Abstract

OBJECTIVE:

To determine the treatment effects of transplanted hepatic progenitor cells (WB-F344 cells) combined with heparin on the acute liver injury in SD rats.

METHODS:

A total of 2*10(7) hepatic stem cells (WB-F344) infected with GFP lentivirus and 8 μL heparin were transplanted through the spleen in SD rats with acute liver injury, which was induced by an intraperitoneal injection of CCl4. The liver and spleen tissues underwent fluorescence examination 1 day after the transplantation. The liver functions were tested, and the liver tissues were histopathologically examined on the 3rd, 7th, 14th, and 28th day of the cell transplantation.

RESULTS:

The transfected WB-F344 cells expressed GFP 3 days after the lentivirus infection and were found in the rat liver 1 day after the WB-F344 transplantation. The liver function and histopathological recovery of the liver tissues in the group of WB-F344 transplantation were better than those of the control group (P<0.05).

CONCLUSION:

Transplantation of hepatic stem cells combined with heparin can promote the liver recovery in rats with acute liver injury induced by CCl4.

• Authors: Zenghui Huang, Shan Zeng, Miao Ouyang, Juanjuan Dong, Yuewen Gong, Hong Shen
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 36 Issue 5 Pg. 411-6 (May 2011) ISSN: 1672-7347 [Print] China
• PMID: 21685696 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Heparin
• Carbon Tetrachloride

Topics

• Animals
• Carbon Tetrachloride
• Carbon Tetrachloride Poisoning
• Heparin (therapeutic use)
• Hepatocytes (transplantation)
• Liver Failure, Acute (chemically induced, therapy)
• Male
• Rats
• Rats, Sprague-Dawley
• Stem Cell Transplantation (methods)