The majority of murine models of
iron sucrose-induced
iron overload were carried out in adult subjects. This cannot reflect the high risk of
iron overload in children who have an increased need for
iron. In this study, we developed four experimental
iron overload models in young rats using
iron sucrose and evaluated different markers of
iron overload, tissue oxidative stress and
inflammation as its consequences.
Iron overload was observed in all
iron-treated rats, as evidenced by significant increases in serum
iron indices, expression of liver
hepcidin gene and total tissue
iron content compared with control rats. We also showed that total tissue
iron content was mainly associated with the dose of
iron whereas serum
iron indices depended essentially on the duration of
iron administration. However, no differences in tissue inflammatory and
antioxidant parameters from controls were observed. Furthermore, only rats exposed to daily
iron injection at a dose of 75 mg/kg
body weight for one week revealed a significant increase in lipid peroxidation in
iron-treated rats compared with their controls. The present results suggest a correlation between
iron overload levels and the dose of
iron, as well as the duration and frequency of
iron injection and confirm that
iron sucrose may not play a crucial role in
inflammation and oxidative stress. This study provides important information about
iron sucrose-induced
iron overload in rats and may be useful for
iron sucrose therapy for
iron deficiency anemia as well as for the prevention and diagnosis of
iron sucrose-induced
iron overload in pediatric patients.