Abstract | OBJECTIVES: METHODS: RESULTS:
Radiotherapy at 3.5 Gy induced a 5.9-fold increase in apoptosis of DU145 cells. Subapoptotic doses of EGCG (1.5-7.5 μM) significantly reduced ionizing radiation-induced apoptosis (P < .001), with the inhibitory effect of EGCG on ionizing radiation being most effective when added 30 minutes before radiotherapy (P < .001). In addition, when radiotherapy and EGCG were used together, an approximate 1.5-fold increase in manganese superoxide dismutase levels was seen compared with the control and a 2-fold increase compared with radiotherapy alone. CONCLUSIONS:
Radiotherapy is effective in inducing apoptosis in DU145 cells, but its effect was significantly reduced in the presence of EGCG, and this was associated with an increase in the induction of manganese superoxide dismutase.
|
Authors | Francis Thomas, Jeff M P Holly, Rajendra Persad, Amit Bahl, Claire M Perks |
Journal | Urology
(Urology)
Vol. 78
Issue 2
Pg. 475.e15-21
(Aug 2011)
ISSN: 1527-9995 [Electronic] United States |
PMID | 21676444
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Antioxidants
- Plant Extracts
- Tea
- Catechin
- epigallocatechin gallate
|
Topics |
- Antioxidants
(pharmacology)
- Catechin
(analogs & derivatives, pharmacology)
- Humans
- Male
- Plant Extracts
(pharmacology)
- Prostatic Neoplasms
(pathology, radiotherapy)
- Tea
- Treatment Outcome
- Tumor Cells, Cultured
(drug effects, radiation effects)
|