Prolonged hepatomegaly in mice that cannot inactivate bacterial endotoxin.

Abstract

UNLABELLED:

Transient hepatomegaly often accompanies acute bacterial infections. Reversible, dose-dependent hepatomegaly also occurs when animals are given intravenous infusions of bacterial lipopolysaccharide (LPS). We found that recovery from LPS-induced hepatomegaly requires a host enzyme, acyloxyacyl hydrolase (AOAH), that inactivates LPS. When we challenged Aoah(-/-) mice with low doses of LPS or gram-negative bacteria, their livers remained enlarged (as much as 80% above normal) many weeks longer than did the livers of Aoah(+/+) animals. When compared with livers from LPS-primed Aoah(+/+) mice, LPS-primed Aoah(-/-) livers had (1) more numerous and larger Kupffer cells, (2) intrasinusoidal leukocyte aggregates and activated sinusoidal endothelial cells, and (3) sustained production of interleukin (IL)-10 and messenger RNAs (mRNAs) for tumor necrosis factor (TNF), IL-10, and IRAK-M. Depleting Kupffer cells decreased the liver enlargement by ≈40%, whereas depletion of neutrophils, dendritic cells, natural killer (NK) cells, NK-T cells, or B cells had no effect. Pretreatment with dexamethasone almost completely prevented prolonged hepatomegaly in Aoah(-/-) mice, whereas neutralizing TNF or interleukin-1β was only partially effective. In contrast, an antagonistic antibody to the IL-10 receptor increased LPS-induced hepatomegaly by as much as 50%.

CONCLUSION:

our findings suggest that persistently active LPS induces Kupffer cells to elaborate mediators that promote the accumulation of leukocytes within enlarged sinusoids. Large increases in IL-10 and several other modulatory molecules are unable to prevent prolonged hepatomegaly in mice that cannot inactivate LPS. The striking findings in this mouse model should encourage studies to find out how AOAH contributes to human liver physiology and disease.

• Authors: Baomei Shao, Richard L Kitchens, Robert S Munford, Thomas E Rogers, Don C Rockey, Alan W Varley
• Journal: Hepatology (Baltimore, Md.) (Hepatology) Vol. 54 Issue 3 Pg. 1051-62 (Sep 02 2011) ISSN: 1527-3350 [Electronic] United States
• PMID: 21674560 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 American Association for the Study of Liver Diseases.

Chemical References

• Cytokines
• Lipopolysaccharides
• Ly96 protein, mouse
• Lymphocyte Antigen 96
• Tlr4 protein, mouse
• Toll-Like Receptor 4
• Nitric Oxide Synthase
• Carboxylic Ester Hydrolases
• acyloxyacyl hydrolase

Topics

• Animals
• Carboxylic Ester Hydrolases (physiology)
• Cell Proliferation
• Cytokines (analysis, physiology)
• Endothelial Cells (physiology)
• Hepatomegaly (etiology, prevention & control)
• Kupffer Cells (physiology)
• Lipopolysaccharides (toxicity)
• Lymphocyte Antigen 96 (physiology)
• Mice
• Nitric Oxide Synthase (physiology)
• Toll-Like Receptor 4 (physiology)