Abstract | BACKGROUND: METHODS: Tissue microarrays were immunohistochemically stained for EBP50, with the staining intensities quantified using automated image analysis software. The data were statistically analyzed using one-way ANOVA with subsequent Tukey tests for multiple comparisons. Eleven cases of NDP, 37 cases of NAC, 15 cases of BPH, 35 cases of HGPIN, 103 cases of PCa, and 36 cases of Mets were analyzed in the microarrays. RESULTS: Specimens of PCa and Mets had the lowest absolute staining for EBP50. Mets staining was significantly lower than NDP (p = 0.027), BPH (p = 0.012), NAC (p < 0.001), HGPIN (p < 0.001), and PCa (p = 0.006). Additionally, HGPIN staining was significantly higher than NAC (p < 0.009) and PCa (p < 0.001). CONCLUSIONS: To our knowledge, this represents the first study comparing the immunohistochemical profiles of EBP50 in PCa and Mets to specimens of HGPIN, BPH, NDP, and NAC and suggests that EBP50 expression is decreased in Mets. Given that PCa also had significantly higher expression than Mets, future studies are warranted to assess EBP50's potential as a prognostic biomarker for prostate cancer.
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Authors | Tanner L Bartholow, Michael J Becich, Uma R Chandran, Anil V Parwani |
Journal | BMC urology
(BMC Urol)
Vol. 11
Pg. 12
(Jun 14 2011)
ISSN: 1471-2490 [Electronic] England |
PMID | 21672215
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Neoplasm Proteins
- Phosphoproteins
- Sodium-Hydrogen Exchangers
- sodium-hydrogen exchanger regulatory factor
|
Topics |
- Adenocarcinoma
(metabolism)
- Aged
- Biomarkers, Tumor
(metabolism)
- Humans
- Male
- Middle Aged
- Neoplasm Proteins
(metabolism)
- Phosphoproteins
(metabolism)
- Prostatic Neoplasms
(metabolism)
- Sodium-Hydrogen Exchangers
(metabolism)
- Tissue Distribution
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