A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar.

Abstract

The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.

• Authors: Ahmet Yilmaz Coban, Yeliz Tanriverdi Cayci, Aydin Deveci, Alper Akgunes, Meltem Uzun, Belma Durupinar
• Journal: Memorias do Instituto Oswaldo Cruz (Mem Inst Oswaldo Cruz) Vol. 106 Issue 3 Pg. 378-80 (May 2011) ISSN: 1678-8060 [Electronic] Brazil
• PMID: 21655831 (Publication Type: Journal Article)

Chemical References

• Antitubercular Agents
• Agar
• Nitrate Reductase
• Isoniazid
• Rifampin

Topics

• Agar
• Antitubercular Agents (pharmacology)
• Drug Resistance, Multiple, Bacterial (drug effects)
• Humans
• Isoniazid (pharmacology)
• Microbial Sensitivity Tests
• Mycobacterium tuberculosis (drug effects)
• Nitrate Reductase (metabolism)
• Predictive Value of Tests
• Rifampin (pharmacology)
• Sensitivity and Specificity
• Tuberculosis, Multidrug-Resistant (microbiology)