Abstract | INTRODUCTION: Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy. Diagnosis and management of PDAC are hampered by the absence of sensitive and specific disease biomarkers. MicroRNAs ( miRNA) are noncoding regulatory RNAs involved in initiation and progression of human cancers. In this study, we sought to determine whether miR-10b could serve as a biomarker for PDAC. EXPERIMENTAL DESIGN:
miRNA expression was characterized by fluorescence-based in situ hybridization using locked nucleic acid-modified DNA probes against miR-10b, miR-21, miR-155, miR-196a, and miR-210, followed by codetection of proteins by immunohistochemistry on the same tissue sections. miRNA expression in surgically resected PDAC tissues and in endoscopic ultrasonography (EUS)-guided fine-needle aspirate (EUS-FNA) samples was analyzed in cytokeratin 19 (CK19)-positive epithelial cells using optical intensity analysis. RESULTS: In 10 resected PDAC samples, miR-10b was the most frequently and consistently overexpressed miRNA among characterized miRNAs, exhibiting a four-fold increase in the cancer cells (P = 0.012). Given this preferential overexpression of miR-10b, we sought to determine whether miR-10b expression was clinically relevant. Accordingly, miR-10b expression was examined in 106 EUS-FNA samples obtained from pancreatic lesions. miR-10b expression was increased in cancer cells compared with CK19-positive epithelial cells in benign lesions (P = 0.0001). In patients with PDACs, lower levels of miR-10b were associated with improved response to multimodality neoadjuvant therapy, likelihood of surgical resection, delayed time to metastasis, and increased survival. CONCLUSION: miR-10b is a novel diagnostic biomarker for PDACs when assessing pancreatic lesions. Expression of miR-10b is predictive of response to neoadjuvant therapy and outcome in this disease.
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Authors | Meir Preis, Timothy B Gardner, Stuart R Gordon, J Marc Pipas, Todd A Mackenzie, Erin E Klein, Daniel S Longnecker, Edward J Gutmann, Lorenzo F Sempere, Murray Korc |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 17
Issue 17
Pg. 5812-21
(Sep 01 2011)
ISSN: 1557-3265 [Electronic] United States |
PMID | 21652542
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | ©2011 AACR. |
Chemical References |
- Biomarkers, Tumor
- MIRN10 microRNA, human
- MIRN155 microRNA, human
- MIRN196 microRNA, human
- MIRN21 microRNA, human
- MIRN210 microRNA, human
- MicroRNAs
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Topics |
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
- Carcinoma, Pancreatic Ductal
(drug therapy, genetics, pathology)
- Female
- Gene Expression Profiling
- Humans
- In Situ Hybridization
- Male
- MicroRNAs
(biosynthesis, genetics)
- Middle Aged
- Neoadjuvant Therapy
- Pancreatic Neoplasms
(drug therapy, genetics, pathology)
- Treatment Outcome
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