No mutation but high mRNA expression of Coxsackie-Adenovirus Receptor was observed in both dilated and ischemic cardiomyopathy.

Abstract

The most common causes of acute myocarditis and the possible consequence of dilated cardiomyopathy are virus infections. The receptor of the two most common viruses connected to these myocardial diseases was identified as Coxsackie-Adenovirus Receptor. The purpose of this study was to assess Coxsackie-Adenovirus Receptor mRNA expression in the myocardium and search for mutations in the Coxsackie-Adenovirus Receptor gene to compare dilated, inflammatory and ischemic cardiomyopathy with control group. All the myocardial samples were obtained from 35 explanted hearts during heart transplantation, than DNA and RNA were isolated from the muscle samples. cDNA was generated from RNA using reverse transcription, and real-time PCR was performed with relative quantification by β-actin gene as endogenous control. Using DNA extracted from the myocardial samples, we sequenced all the seven exons of the Coxsackie-Adenovirus Receptor gene. Coxsackie-Adenovirus Receptor mRNA expression was higher in both ischemic and dilated cardiomyopathy groups than in inflammatory cardiomyopathy and healthy control groups. Sequencing of CAR gene showed no sign of mutation. Therefore, the sequences result of CAR exons did not show any mutation or polymorphism, that explains a determinant role of CAR in dilated or ischemic CM. Our results suggest that high mRNA expression of Coxsackie-Adenovirus Receptor may support its role in regeneration of the damaged myocardium rather than having any role in viral mediated heart disease.

• Authors: Eniko Tatrai, Katalin Bedi, Ilona Kovalszky, Istvan Hartyanszky, Andras Laszik, Gyorgy Acsady, Peter Sotonyi, Marta Hubay
• Journal: Forensic science international (Forensic Sci Int) Vol. 212 Issue 1-3 Pg. 47-50 (Oct 10 2011) ISSN: 1872-6283 [Electronic] Ireland
• PMID: 21641134 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• CLMP protein, human
• Coxsackie and Adenovirus Receptor-Like Membrane Protein
• DNA, Viral
• RNA, Messenger
• Receptors, Virus

Topics

• Adolescent
• Adult
• Cardiomyopathy, Dilated (pathology, virology)
• Case-Control Studies
• Coxsackie and Adenovirus Receptor-Like Membrane Protein
• Coxsackievirus Infections (diagnosis, pathology)
• DNA, Viral (analysis)
• Female
• Forensic Medicine (methods)
• Gene Expression Regulation, Viral
• Humans
• Male
• Middle Aged
• Mutation
• Myocardial Ischemia (pathology, virology)
• Myocarditis (pathology, virology)
• Myocardium (pathology)
• RNA, Messenger (analysis)
• Real-Time Polymerase Chain Reaction
• Receptors, Virus (genetics, metabolism)
• Reference Values
• Up-Regulation
• Young Adult