[Vasoactive intestinal peptide receptor in a human pancreatic carcinoma cell line].

Abstract

Vasoactive intestinal peptide (VIP) receptors were identified in a human pancreatic carcinoma cell line by radioreceptor assay, including time course, dissociation study, competitive inhibition, and cross reactions with secretin and glucagon, both of which are hormones of the same family. Peak binding of 125I-VIP to the cells occurred at 20-30 min at 37 degrees C. Displacement curve showed an increasing inhibition of binding with increasing concentration of unlabeled VIP(inhibited by 95% at 1 microM of VIP). KD of VIP receptors was 1.68 x 10(-10)M, and the number of binding sites was 3.6 x 10(5)/cell. It was also shown that VIP was able to induce cAMP production in this cell line, indicating that the VIP receptors in this cell line were biologically active.

• Authors: Y Chen
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 70 Issue 3 Pg. 135-7, 12 (Mar 1990) ISSN: 0376-2491 [Print] China
• PMID: 2163737 (Publication Type: English Abstract, Journal Article)

Chemical References

• Receptors, Gastrointestinal Hormone
• Receptors, Vasoactive Intestinal Peptide
• Vasoactive Intestinal Peptide

Topics

• Carcinoma, Intraductal, Noninfiltrating (analysis)
• Humans
• Pancreatic Neoplasms (analysis)
• Receptors, Gastrointestinal Hormone (analysis)
• Receptors, Vasoactive Intestinal Peptide
• Tumor Cells, Cultured (analysis)
• Vasoactive Intestinal Peptide (metabolism)