Development and application of an improved infectivity assay for the standardization of avian encephalomyelitis vaccines.

Abstract

An improved infectivity assay for avian encephalomyelitis viruses (AEVs) is described in which susceptible 7-day-old chicken embryos were inoculated with dilutions of a particular AEV strain and, after incubation for 12 days, the presence of viral antigen in brains was detected by an enzyme-linked immunosorbent assay (ELISA). The assay (the brain antigen ELISA), which may be used for the titration of both egg-adapted and non-egg-adapted strains, can be completed within 12 days. For the conventional essays of non-egg-adapted vaccine strains, embryos are infected in ovo and hatched chickens examined for neurological signs after 3-4 weeks. No animal handling facilities are required with the brain antigen ELISA and results of comparable sensitivity to the hatch-out assay were obtained.

• Authors: D R Shafren, G A Tannock
• Journal: Vaccine (Vaccine) Vol. 8 Issue 3 Pg. 283-5 (Jun 1990) ISSN: 0264-410X [Print] Netherlands
• PMID: 2163576 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Viral Vaccines

Topics

• Animals
• Antigens, Viral (analysis)
• Biological Assay
• Brain (immunology)
• Chick Embryo
• Encephalomyelitis Virus, Avian (immunology)
• Enterovirus (immunology)
• Enterovirus Infections (prevention & control)
• Enzyme-Linked Immunosorbent Assay
• Viral Vaccines (analysis, standards)