[Methylation profiling of the cell cycle regulating genes in placenta of human embryos with chromosomal mosaicism].

Abstract

To date the investigation of epigenetic mechanisms of cell cycle regulation, ensuring genomic stability maintaining and accurate transfer of hereditary information to daughter cells, is of a considerable interest. Development of the up-to-date molecular technologies allows determining methylation pattern of the whole genome at a single round of analysis. In this work for the first time the epigenetic status of placental tissues of human embryos with mosaic karyotype was studied using the genome-wide Illumina Infinium HumanMethylation27 BeadChip Array (Illumina, USA). The groups of genes, related to the cell cycle and its regulation and containing differentially methylated CpG-sites in their promoter regions, are determined. The methylation level of oncogenes (ARHGEF1, RGF5), tumor-suppressors (APC2, BRACA2, DCC, GRLF1, RB1, TP73, TSPYL2, VHL) as well as genes, participating in chromosome segregation regulation (CNTROB, GMNN, PROCR, TACC1), was changed most frequently.

• Authors: A A Kashevarova, E N Tolmacheva, E A Sazhenova, N N Sikhanova, I N Lebedev
• Journal: Molekuliarnaia biologiia (Mol Biol (Mosk)) 2011 Mar-Apr Vol. 45 Issue 2 Pg. 316-24 ISSN: 0026-8984 [Print] Russia (Federation)
• PMID: 21634119 (Publication Type: English Abstract, Journal Article)

Chemical References

• Cell Cycle Proteins

Topics

• Cell Cycle (genetics)
• Cell Cycle Proteins (genetics)
• CpG Islands (genetics)
• DNA Methylation
• Epigenomics
• Female
• Genes, Tumor Suppressor
• Humans
• Microarray Analysis
• Mosaicism (embryology)
• Oncogenes (genetics)
• Placenta (cytology, metabolism)
• Pregnancy