Frequent expression of receptors for granulocyte-macrophage colony-stimulating factor on human nonhematopoietic tumor cell lines.

Abstract

Receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) were identified on 9 of 35 (26%) human nonhematopoietic tumor cell lines including non-small cell lung cancer, stomach cancer, colon cancer, and osteosarcoma cells. GM-CSF receptors distributed on these human tumor cells were low affinity types with an equilibrium dissociation constant of 1.5-10.0 nM. Cross-linking studies revealed that the molecular weights of the low affinity GM-CSF receptors were 65-85 kilodaltons. The high affinity receptors identified on hematopoietic cells were not detected on human nonhematopoietic tumor cells which we studied, and we could detect no effects of GM-CSF on cell growth of these tumor cells.

• Authors: K Miyagawa, S Chiba, K Shibuya, Y F Piao, S Matsuki, J Yokota, M Terada, K Miyazono, F Takaku
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 143 Issue 3 Pg. 483-7 (Jun 1990) ISSN: 0021-9541 [Print] United States
• PMID: 2162848 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Cell Surface
• Receptors, Colony-Stimulating Factor

Topics

• Carcinoma, Non-Small-Cell Lung (metabolism, pathology, ultrastructure)
• Cell Transformation, Neoplastic (metabolism, pathology)
• Colonic Neoplasms (metabolism, pathology, ultrastructure)
• Gene Expression (physiology)
• Humans
• Lung Neoplasms (metabolism, pathology, ultrastructure)
• Osteosarcoma (metabolism, pathology, ultrastructure)
• Receptors, Cell Surface (genetics, metabolism, physiology)
• Receptors, Colony-Stimulating Factor
• Stomach Neoplasms (metabolism, pathology, ultrastructure)
• Tumor Cells, Cultured (metabolism, pathology, ultrastructure)