Effect of gestational hypercholesterolemia and maternal immunization on offspring plasma eicosanoids.

Abstract

OBJECTIVE:

Maternal immunization with oxidized low-density lipoprotein prior to pregnancy prevents pathogenic in utero programming by gestational hypercholesterolemia, but it is unknown whether gestational hypercholesterolemia and maternal immunization affect similar pathways.

STUDY DESIGN:

A lipidomic approach was used for unbiased plasma eicosanoid profiling in adult offspring of immunized and nonimmunized normocholesterolemic or hypercholesterolemic rabbit mothers.

RESULTS:

Gestational hypercholesterolemia was associated with increased levels of some eicosanoids formed by the cyclooxygenase and 12-lipoxygenase pathways only (including thromboxane B2, prostaglandin [PG] F2α, PGE2, and PGD2). Immunization of hypercholesterolemic or normocholesterolemic mothers reduced 9 of 14 eicosanoids of the cyclooxygenase pathway, 21 of 23 eicosanoids of the 5- and 12-lipoxygenase pathways (eg, 5-hydroxyeicosatetraenoic acid, hepoxilin B3, 12-hydroxyeicosatetraenoic acid), 8 of 19 eicosanoids of the cytochrome P-450 pathway, and all metabolites of the nonenzymatic pathway.

CONCLUSION:

Maternal immunization not only counteracts in utero programming by gestational hypercholesterolemia but reduces a broad range of eicosanoid modulators of immunity and inflammation in offspring.

• Authors: Oswald Quehenberger, Tomoya Yamashita, Aaron M Armando, Edward A Dennis, Wulf Palinski
• Journal: American journal of obstetrics and gynecology (Am J Obstet Gynecol) Vol. 205 Issue 2 Pg. 156.e15-25 (Aug 2011) ISSN: 1097-6868 [Electronic] United States
• PMID: 21621186 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Mosby, Inc. All rights reserved.

Chemical References

• Eicosanoids
• Lipoproteins, LDL
• oxidized low density lipoprotein
• Prostaglandin-Endoperoxide Synthases

Topics

• Animals
• Animals, Newborn
• Eicosanoids (analysis, metabolism)
• Female
• Hypercholesterolemia (immunology, physiopathology)
• Immunization (methods)
• Lipoproteins, LDL (pharmacology)
• Pregnancy
• Pregnancy Complications, Cardiovascular (diagnosis, immunology)
• Pregnancy, Animal
• Prostaglandin-Endoperoxide Synthases (metabolism)
• Rabbits
• Reference Values
• Sensitivity and Specificity