Histological analysis of esophageal muscular layers from 27 autopsy cases with mixed connective tissue disease (MCTD).

Abstract

Esophageal symptoms in mixed connective tissue disease (MCTD) have been investigated radiologically. We investigated the esophageal lesions in MCTD histopathologically, and analyzed relationships between these lesions and autoantibodies extracted from the serum of MCTD patients. Esophageal tissues from 27 MCTD patients submitted to autopsy were examined. We compared histopathological features of the esophagus in different wall layers from the mucosa, submucosa, and muscular layer to the adventitia, and in the upper, middle, and lower portions of esophagus. The most striking change observed was severe atrophy and occasional loss of smooth muscle cells in the muscular layer, followed by fibrosis. These muscular changes were particularly prominent in the inner layer of the lower esophagus. Immunohistochemically, degenerated muscular tissues of the esophagus were positive for anti-IgG and anti-C3 antibodies, but not for anti-IgM antibodies. IgG fractions extracted from three MCTD patients were immunohistochemically used to examine whether some antibodies in MCTD patients showed reactivity for esophageal components. The IgG fractions isolated from MCTD patients reacted with smooth muscle from non-connective tissue disease cases, suggesting that some serum antibodies may trigger esophageal changes. These findings suggest that esophageal lesions associated with clinical dysphagia in MCTD may be related to autoantibodies.

• Authors: Miwa Uzuki, Akihisa Kamataki, Mika Watanabe, Nobuhito Sasaki, Yasuhiro Miura, Takashi Sawai
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 207 Issue 6 Pg. 383-90 (Jun 15 2011) ISSN: 1618-0631 [Electronic] Germany
• PMID: 21620578 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier GmbH. All rights reserved.

Chemical References

• Autoantibodies

Topics

• Adolescent
• Adult
• Aged
• Atrophy
• Autoantibodies (blood)
• Autopsy
• Deglutition Disorders (immunology, pathology)
• Esophageal Motility Disorders (immunology, pathology)
• Esophagus (immunology, pathology)
• Female
• Fibrosis
• Humans
• Immunohistochemistry
• Japan
• Male
• Middle Aged
• Mixed Connective Tissue Disease (immunology, pathology)
• Mucous Membrane (pathology)
• Muscle, Smooth (immunology, pathology)
• Young Adult