Cyclic AMP-dependent expression of the heavy neurofilament (NF-H) polypeptide in differentiating neuroblastoma cells.

Abstract

Dibutyryl cyclic AMP (dbcAMP) treatment of Neuro 2A neuroblastoma cells induces cell differentiation and neurite outgrowth. Undifferentiated cells express the heavy neurofilament polypeptide (NF-H) at a low level. Following differentiation, there is a large increase in its expression due to an increase in the corresponding cellular mRNA. A similar increase is seen following forskolin treatment of the cells indicating that the increase in NF-H expression is cyclic AMP-dependent. The increase in mRNA is due to an increase in gene transcription as demonstrated by a nuclear run-off assay.

• Authors: K C Breen, B H Anderton
• Journal: Brain research. Molecular brain research (Brain Res Mol Brain Res) Vol. 7 Issue 2 Pg. 161-5 (Feb 1990) ISSN: 0169-328X [Print] Netherlands
• PMID: 2160043 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intermediate Filament Proteins
• Neurofilament Proteins
• Bucladesine

Topics

• Animals
• Bucladesine (pharmacology)
• Cell Differentiation
• Gene Expression Regulation (drug effects)
• Intermediate Filament Proteins (genetics, metabolism)
• Mice
• Neuroblastoma
• Neurofilament Proteins
• Tumor Cells, Cultured (drug effects, metabolism)