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Identification of monoclonal antibodies capable of differentiating antigenic varieties of eastern equine encephalitis viruses.

Abstract
We have isolated and characterized 3 monoclonal antibody (Mab) reagents useful in the serological identification of varieties of eastern equine encephalitis (EEE) viruses. These antibodies were specific for the E1 glycoprotein of their homologous viruses. One Mab, 1B5C-3, reacted specifically with all North American (NA) EEE viruses isolated over a 50 year period. This antigenic stability of NA isolates was genetically confirmed by oligonucleotide fingerprinting. Evolutionary stability is a unique feature among alphaviruses. The Mab, 1C1J-4 reacted specifically with 1 South American isolate of EEE virus. A third Mab, 1B1C-4, was EEE virus complex reactive. While none of these antibodies had virus neutralizing activity, the identified reactivities could be demonstrated in the more rapid serological tests of enzyme-linked immunosorbent assay and indirect immunofluorescence.
AuthorsJ T Roehrig, A R Hunt, G J Chang, B Sheik, R A Bolin, T F Tsai, D W Trent
JournalThe American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 42 Issue 4 Pg. 394-8 (Apr 1990) ISSN: 0002-9637 [Print] United States
PMID2158755 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral
  • Oligonucleotides
Topics
  • Alphavirus (immunology)
  • Animals
  • Antibodies, Monoclonal (immunology)
  • Antibodies, Viral (immunology)
  • Antigenic Variation
  • Antigens, Viral (analysis)
  • Cross Reactions
  • Encephalitis Virus, Eastern Equine (immunology, isolation & purification)
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Hemagglutination Inhibition Tests
  • Hybridomas
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Nucleotide Mapping
  • Oligonucleotides (analysis)

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