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Type V collagen-induced upregulation of capn2 (large subunit of m-calpain) gene expression and DNA fragmentation in 8701-BC breast cancer cells.

Abstract
Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
AuthorsClaudio Luparello, Rosalia Sirchia
JournalBiological chemistry (Biol Chem) Vol. 392 Issue 6 Pg. 501-4 (Apr 2011) ISSN: 1437-4315 [Electronic] Germany
PMID21585285 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Collagen Type V
  • DNA, Neoplasm
  • Calpain
  • CAPN2 protein, human
Topics
  • Breast Neoplasms (enzymology, metabolism, pathology)
  • Calpain (genetics)
  • Cell Line, Tumor
  • Collagen Type V (pharmacology)
  • DNA Fragmentation (drug effects)
  • DNA, Neoplasm (metabolism)
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic (drug effects, genetics)
  • Humans
  • Polymerase Chain Reaction
  • Up-Regulation (drug effects)

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