Abstract |
Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
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Authors | Claudio Luparello, Rosalia Sirchia |
Journal | Biological chemistry
(Biol Chem)
Vol. 392
Issue 6
Pg. 501-4
(Apr 2011)
ISSN: 1437-4315 [Electronic] Germany |
PMID | 21585285
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Collagen Type V
- DNA, Neoplasm
- Calpain
- CAPN2 protein, human
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Topics |
- Breast Neoplasms
(enzymology, metabolism, pathology)
- Calpain
(genetics)
- Cell Line, Tumor
- Collagen Type V
(pharmacology)
- DNA Fragmentation
(drug effects)
- DNA, Neoplasm
(metabolism)
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Enzymologic
(drug effects, genetics)
- Humans
- Polymerase Chain Reaction
- Up-Regulation
(drug effects)
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