Abstract | PURPOSE: METHODS: Male Wistar rats were randomized into four groups and treated as follows: (1) saline solution (control); (2) zymosan; (3) HBO 4 and 11 h after zymosan injection; (4) HBO 4 and 11 h after saline solution injection. Zymosan-induced damage of the lungs, liver, and small intestine was evaluated using histology and biochemistry. The activation of the TLR signaling pathway was measured with Western blot, reverse transcriptase polymerase chain reaction analysis (RT-PCR), and immunohistochemistry. RESULTS:
Zymosan induced a severe inflammatory response characterized by the activation of the TLR signaling pathway and by an organ dysfunction. HBO exposure significantly reduced the development of lung, liver, and intestine injury in our experimental model. It also significantly reduced the zymosan-induced expression of TLR2 and TLR4, NF-κB activation, and cytokine production. CONCLUSIONS: Taken together, these results suggest that, by interfering with the TLR pathway, HBO treatment may exert a protective effect against tissue injury caused by zymosan-induced generalized inflammation.
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Authors | Barbara Rinaldi, Salvatore Cuzzocrea, Maria Donniacuo, Annalisa Capuano, Donatella Di Palma, Francesco Imperatore, Emanuela Mazzon, Rosanna Di Paola, Loredana Sodano, Francesco Rossi |
Journal | Intensive care medicine
(Intensive Care Med)
Vol. 37
Issue 7
Pg. 1110-9
(Jul 2011)
ISSN: 1432-1238 [Electronic] United States |
PMID | 21567111
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-1beta
- Toll-Like Receptors
- Tumor Necrosis Factor-alpha
- Zymosan
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Topics |
- Analysis of Variance
- Animals
- Blotting, Western
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Hyperbaric Oxygenation
- Immunohistochemistry
- Interleukin-1beta
(metabolism)
- Male
- Multiple Organ Failure
(metabolism, therapy)
- Random Allocation
- Rats
- Rats, Wistar
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
- Toll-Like Receptors
(metabolism)
- Tumor Necrosis Factor-alpha
(metabolism)
- Zymosan
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