Abstract | BACKGROUND AND AIMS: METHODS: RESULTS: This study demonstrates that acute hypoxia inhibits electrogenic chloride secretion via AMPK mediated inhibition of CFTR. Pre-treatment of tissues with the AMPK inhibitor 6-[4-(2-piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo [1,5-a] pyrimidine (compound C) in part reversed the effects of acute hypoxia on chloride secretion. CONCLUSION: We therefore suggest that AMPK is a key component of the adaptive cellular response to mucosal hypoxia in the colon. Furthermore, AMPK may represent a potential therapeutic target in diseased states or in prevention of ischemic intestinal injury.
|
Authors | Danielle Collins, Sascha Kopic, Julia Bachlechner, Markus Ritter, Desmond C Winter, John P Geibel |
Journal | Annals of surgery
(Ann Surg)
Vol. 254
Issue 6
Pg. 957-63
(Dec 2011)
ISSN: 1528-1140 [Electronic] United States |
PMID | 21562404
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Chloride Channels
- Chlorides
- Cystic Fibrosis Transmembrane Conductance Regulator
- AMP-Activated Protein Kinases
|
Topics |
- AMP-Activated Protein Kinases
(physiology)
- Animals
- Cell Hypoxia
(physiology)
- Chloride Channels
(physiology)
- Chlorides
(metabolism)
- Colon
(blood supply)
- Cystic Fibrosis Transmembrane Conductance Regulator
(antagonists & inhibitors)
- Humans
- Intestinal Mucosa
(blood supply)
- Ischemia
(physiopathology)
- Male
- Membrane Potentials
(physiology)
- Microscopy, Fluorescence
- Rats
- Rats, Sprague-Dawley
|